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human adipose derived mesenchymal stem cells asc (ATCC)

Name: human adipose derived mesenchymal stem cells asc
Brand: ATCC
Rating: 97 (241 reviews)

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ATCC human adipose derived mesenchymal stem cells asc
Human Adipose Derived Mesenchymal Stem Cells Asc, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 241 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human adipose derived mesenchymal stem cells asc/product/ATCC
Average 97 stars, based on 241 article reviews

human adipose derived mesenchymal stem cells asc - by Bioz Stars, 2026-03

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Effect of Rab7 inhibition on the viability and morphology of ASCs. (A) Viability of ASCs after treatment with different concentrations of CID-1067700 at 24 and 72 hours. No significant changes in cell viability were observed. (B) Representative bright-field images illustrating the morphological changes in ASCs after 10 days of treatment with differentiation media (DM) with and without 40 μM CID-1067700, in comparison to undifferentiated ASCs. Scale bars = 100 μm.

Journal: Frontiers in Immunology

Article Title: Rab7 inhibitor enhances stem cell differentiation into keratinocyte-like cells with anti-inflammatory properties

doi: 10.3389/fimmu.2025.1503007

Figure Lengend Snippet: Effect of Rab7 inhibition on the viability and morphology of ASCs. (A) Viability of ASCs after treatment with different concentrations of CID-1067700 at 24 and 72 hours. No significant changes in cell viability were observed. (B) Representative bright-field images illustrating the morphological changes in ASCs after 10 days of treatment with differentiation media (DM) with and without 40 μM CID-1067700, in comparison to undifferentiated ASCs. Scale bars = 100 μm.

Article Snippet: The adipose-derived mesenchymal stem cell (ASCs) line, ASC52telo (SCRC-4000, ATCC) immortalized with human telomerase reverse transcriptase (hTERT), were selected for their consistent stem cell properties and reproducibility.

Techniques: Inhibition, Comparison

Rab7 inhibition leads to transcriptomic changes in ASCs. (A) Principal component analysis (PCA) of the transcriptomic profiles of cells treated as control (DM), with vehicle (DMSO), or with CID. PCA revealed clustering of the vehicle group with DM group, indicating a high degree of similarity between these two groups. (B) Volcano plot showed the differentially expressed genes (DEGs) in the CID-treated ASCs compared to those treated with DM. Downregulated genes are shown in blue, upregulated genes are in red, while insignificantly regulated genes are in grey.

Journal: Frontiers in Immunology

Article Title: Rab7 inhibitor enhances stem cell differentiation into keratinocyte-like cells with anti-inflammatory properties

doi: 10.3389/fimmu.2025.1503007

Figure Lengend Snippet: Rab7 inhibition leads to transcriptomic changes in ASCs. (A) Principal component analysis (PCA) of the transcriptomic profiles of cells treated as control (DM), with vehicle (DMSO), or with CID. PCA revealed clustering of the vehicle group with DM group, indicating a high degree of similarity between these two groups. (B) Volcano plot showed the differentially expressed genes (DEGs) in the CID-treated ASCs compared to those treated with DM. Downregulated genes are shown in blue, upregulated genes are in red, while insignificantly regulated genes are in grey.

Techniques: Inhibition, Control

Impact of Rab7 inhibition on signalling pathways, biological processes, and upstream regulators in ASCs. (A) Chord Diagram of the top six significantly enriched pathways. (B) The top five enriched GO biological processes among the DEGs in the CID-treated ASCs. (C) Predicted activated or (D) inhibited upstream regulators for the DEGs in CID-treated ASCs compared to the control group. These predictions were made using the iPathwayGuide software.

Journal: Frontiers in Immunology

Article Title: Rab7 inhibitor enhances stem cell differentiation into keratinocyte-like cells with anti-inflammatory properties

doi: 10.3389/fimmu.2025.1503007

Figure Lengend Snippet: Impact of Rab7 inhibition on signalling pathways, biological processes, and upstream regulators in ASCs. (A) Chord Diagram of the top six significantly enriched pathways. (B) The top five enriched GO biological processes among the DEGs in the CID-treated ASCs. (C) Predicted activated or (D) inhibited upstream regulators for the DEGs in CID-treated ASCs compared to the control group. These predictions were made using the iPathwayGuide software.

Techniques: Inhibition, Control, Software

Validation of the expression levels of the differentially expressed genes identified by microarray with qPCR. (A) Bar chart displaying the relative expression levels of seven selected DEGs ( COL81A, COL1A1, ITGB8, MGP, FGF7, VCAM1, and HMOX-1) . Bars represent the log2-fold changes in gene expression in CID-1067700-treated ASCs (CID) compared to the control group (DM). (B) Validation of the expression levels of the selected genes by qPCR. The expression levels are normalized to GAPDH and calculated relative to the expression of ASCs treated with DM only using the 2 -∆∆Cq method. Data are presented as mean ± standard deviation (SD) of three experimental replicates. The vehicle control was added to the qPCR to validate similarity with the negative control.

Journal: Frontiers in Immunology

Article Title: Rab7 inhibitor enhances stem cell differentiation into keratinocyte-like cells with anti-inflammatory properties

doi: 10.3389/fimmu.2025.1503007

Figure Lengend Snippet: Validation of the expression levels of the differentially expressed genes identified by microarray with qPCR. (A) Bar chart displaying the relative expression levels of seven selected DEGs ( COL81A, COL1A1, ITGB8, MGP, FGF7, VCAM1, and HMOX-1) . Bars represent the log2-fold changes in gene expression in CID-1067700-treated ASCs (CID) compared to the control group (DM). (B) Validation of the expression levels of the selected genes by qPCR. The expression levels are normalized to GAPDH and calculated relative to the expression of ASCs treated with DM only using the 2 -∆∆Cq method. Data are presented as mean ± standard deviation (SD) of three experimental replicates. The vehicle control was added to the qPCR to validate similarity with the negative control.

Techniques: Biomarker Discovery, Expressing, Microarray, Gene Expression, Control, Standard Deviation, Negative Control

Journal: Frontiers in Immunology

Article Title: Rab7 inhibitor enhances stem cell differentiation into keratinocyte-like cells with anti-inflammatory properties

doi: 10.3389/fimmu.2025.1503007

Figure Lengend Snippet: Rab7 Inhibition induces changes in the proteomic profile of ASCs. The upper left image shows the original scan of the protein array membrane. Noticeable reduction in the levels of inflammatory cytokines IL-1α, IL-8, IL-17A, and IL-32 were observed in CID-treated cells, whereas the expression level of EGF was increased. Bars represent the mean fold change in protein expression ± standard deviation (SD) of two experimental replicates in response to CID treatment compared to the control (DM).

Techniques: Inhibition, Protein Array, Membrane, Expressing, Standard Deviation, Control

Rab7 Inhibition enhances the expression of epithelial differentiation markers in ASCs. The latter were cultured with differentiation media for ten days with and without CID-1067700, and immunocytochemistry analysis was performed to assess the expression of Cytokeratin 5 and 14, as well as Filaggrin and P63. Nuclei were counterstained with DAPI (blue). The bars indicate the mean fluorescence intensity unit (FU) per cell ± SD of 3 replicates. Asterisks represent significant difference compared to cells treated with differentiation media (DM) only (* p<0.5, ** p<0.01). Scale bars = 100 μm.

Journal: Frontiers in Immunology

Article Title: Rab7 inhibitor enhances stem cell differentiation into keratinocyte-like cells with anti-inflammatory properties

doi: 10.3389/fimmu.2025.1503007

Figure Lengend Snippet: Rab7 Inhibition enhances the expression of epithelial differentiation markers in ASCs. The latter were cultured with differentiation media for ten days with and without CID-1067700, and immunocytochemistry analysis was performed to assess the expression of Cytokeratin 5 and 14, as well as Filaggrin and P63. Nuclei were counterstained with DAPI (blue). The bars indicate the mean fluorescence intensity unit (FU) per cell ± SD of 3 replicates. Asterisks represent significant difference compared to cells treated with differentiation media (DM) only (* p<0.5, ** p<0.01). Scale bars = 100 μm.

Techniques: Inhibition, Expressing, Cell Culture, Immunocytochemistry, Fluorescence

Effect of Rab7 inhibition on the expression of keratinocyte markers in ASCs cells. The gene expression of (A) Vimentin, (B) Filaggrin, (C) Cytokeratin 5, and (D) Cytokeratin 14 was measured by Real-time PCR. The expression levels were normalized to GAPDH expression and calculated relative to cells treated with differentiation media (DM) alone. Asterisks indicate a significant difference compared to cells treated with DM (* p<0.05, ** p<0.01, *** p<0.001).

Journal: Frontiers in Immunology

Article Title: Rab7 inhibitor enhances stem cell differentiation into keratinocyte-like cells with anti-inflammatory properties

doi: 10.3389/fimmu.2025.1503007

Figure Lengend Snippet: Effect of Rab7 inhibition on the expression of keratinocyte markers in ASCs cells. The gene expression of (A) Vimentin, (B) Filaggrin, (C) Cytokeratin 5, and (D) Cytokeratin 14 was measured by Real-time PCR. The expression levels were normalized to GAPDH expression and calculated relative to cells treated with differentiation media (DM) alone. Asterisks indicate a significant difference compared to cells treated with DM (* p<0.05, ** p<0.01, *** p<0.001).

Techniques: Inhibition, Expressing, Gene Expression, Real-time Polymerase Chain Reaction

Human ASC osteogenesis is supported by culture on SBG-PLGA composites and rhBMP-2 treatment. mRNA levels of the early and late osteoblastic markers, BMP-2 and Noggin ( NOG ) in human ASCs cultured on SBG-PLGA composites in ( A ), ( C ) standard osteogenic medium or ( B ), ( D ) standard osteogenic medium supplemented with 100 ng/ml rhBMP-2. Results are presented as relative mRNA expression levels vs. mRNA levels for ASCs cultured on a plain PLGA control (black line at 1). ( E ) Nitric oxide (NO) concentration in culture media after 24-h culture of ASC cells on SBG-PLGA composites in standard osteogenic medium. Averages ± SD are indicated. One-way or two-way ANOVA tests, * p < 0.05, ** p < 0.001, *** p < 0.0001 relative to the PLGA control group

Journal: Journal of Biological Engineering

Article Title: Rapid osteoinduction of human adipose-derived stem cells grown on bioactive surfaces and stimulated by chemically modified media flow

doi: 10.1186/s13036-025-00491-2

Figure Lengend Snippet: Human ASC osteogenesis is supported by culture on SBG-PLGA composites and rhBMP-2 treatment. mRNA levels of the early and late osteoblastic markers, BMP-2 and Noggin ( NOG ) in human ASCs cultured on SBG-PLGA composites in ( A ), ( C ) standard osteogenic medium or ( B ), ( D ) standard osteogenic medium supplemented with 100 ng/ml rhBMP-2. Results are presented as relative mRNA expression levels vs. mRNA levels for ASCs cultured on a plain PLGA control (black line at 1). ( E ) Nitric oxide (NO) concentration in culture media after 24-h culture of ASC cells on SBG-PLGA composites in standard osteogenic medium. Averages ± SD are indicated. One-way or two-way ANOVA tests, * p < 0.05, ** p < 0.001, *** p < 0.0001 relative to the PLGA control group

Article Snippet: ASC52telo cells (ASC; ATCC, SCRC-4000) and normal human ASCs (ATCC, PCS-500-011) were expanded in the dedicated medium (ATCC, Mesenchymal Stem Cell Basal Medium with Mesenchymal Stem Cell Growth Kit and G418).

Techniques: Cell Culture, Expressing, Control, Concentration Assay

Cumulative osteogenic effect of Phenamil and PD98059 treatment in rhBMP-2 stimulated human ASCs cultured on SBG-PLGA composites. mRNA levels of osteoblastic markers in ( A ) 7-day and ( B ) 21-day ASC cultures on SBG-PLGA composites. ASCs were cultured in osteogenic medium supplemented with 100 ng/ml rhBMP-2 or 100 ng/ml rhBMP-2, 50 µM PD98059 and 20 µM Phenamil. Results are presented as relative mRNA expression compared to mRNA levels in control cells cultured on PLGA with rhBMP-2 only (marked as black line at 1). ( C ) mRNA levels of selected osteoblastic markers in 3-day osteogenic ASC cultures treated with different doses of rhBMP-2 (25–250 ng/ml), Phenamil (5–50 µM) or PD98059 (1-125 µM); under fluid shear stress. Results are presented as the expression relative to osteogenic cultures treated only with ascorbic acid, dexamethasone and β-glycerophosphate. ( D ) Graphical hypothesis of BMP-2, PD98059 and Phenamil cross-talk in intracellular signaling. Average values ± SD are indicated. Two-way ANOVA test, * p < 0.05, ** p < 0.001, *** p < 0.0001 relative to the PLGA control group or between marked groups. BMP-2 – bone morphogenetic protein 2, OC – osteocalcin, ON – osteonectin, FOS – AP-1 transcription factor subunit (c-fos), OPG – osteoprotegerin

Journal: Journal of Biological Engineering

Article Title: Rapid osteoinduction of human adipose-derived stem cells grown on bioactive surfaces and stimulated by chemically modified media flow

doi: 10.1186/s13036-025-00491-2

Figure Lengend Snippet: Cumulative osteogenic effect of Phenamil and PD98059 treatment in rhBMP-2 stimulated human ASCs cultured on SBG-PLGA composites. mRNA levels of osteoblastic markers in ( A ) 7-day and ( B ) 21-day ASC cultures on SBG-PLGA composites. ASCs were cultured in osteogenic medium supplemented with 100 ng/ml rhBMP-2 or 100 ng/ml rhBMP-2, 50 µM PD98059 and 20 µM Phenamil. Results are presented as relative mRNA expression compared to mRNA levels in control cells cultured on PLGA with rhBMP-2 only (marked as black line at 1). ( C ) mRNA levels of selected osteoblastic markers in 3-day osteogenic ASC cultures treated with different doses of rhBMP-2 (25–250 ng/ml), Phenamil (5–50 µM) or PD98059 (1-125 µM); under fluid shear stress. Results are presented as the expression relative to osteogenic cultures treated only with ascorbic acid, dexamethasone and β-glycerophosphate. ( D ) Graphical hypothesis of BMP-2, PD98059 and Phenamil cross-talk in intracellular signaling. Average values ± SD are indicated. Two-way ANOVA test, * p < 0.05, ** p < 0.001, *** p < 0.0001 relative to the PLGA control group or between marked groups. BMP-2 – bone morphogenetic protein 2, OC – osteocalcin, ON – osteonectin, FOS – AP-1 transcription factor subunit (c-fos), OPG – osteoprotegerin

Techniques: Cell Culture, Expressing, Control, Shear

Fluid shear stress strengthens the osteogenic effects of rhBMP-2, PD98059 and Phenamil in ASCs cultured on SBG-PLGA composites. mRNA levels of osteoblastic markers after 7-day ASC culture on SBG-PLGA composites in ( A ) standard osteogenic medium under either static conditions or with fluid shear stress; and ( C ) osteogenic medium supplemented with 100 ng/ml rhBMP-2, 50 µM PD98059 and 20 µM Phenamil under either static conditions or with fluid shear stress. Results are presented as relative mRNA expression levels compared to mRNA levels in a control, static culture on PLGA (marked as a black line at 1). ( B ) The method of fluid shear stress application in ASC cultures using a standard laboratory see-saw rocker (7° tilt angle, 6 RPM frequency). ( D ) F-actin distribution in ASCs (Phalloidin-Atto488, magenta colored) at day 3 of culture in osteogenic medium supplemented with rhBMP-2, PD98059 and Phenamil after continuous static or dynamic culture conditions applied for 3 days. Scale bar represents 100 μm. ( E ) Western blot (WB) analysis of p-ERK1/2 and p-SMAD1/5/8 in ASCs after 1-h treatment with rhBMP-2 or rhBMP-2, PD98059 and Phenamil in static or dynamic conditions (upper panel) along with densitometric quantifications of WB results normalized to GAPDH levels. Averages ± SD are indicated. Two-way ANOVA test, * p < 0.05, ** p < 0.001, *** p < 0.0001 relative to the static PLGA control or between marked groups

Journal: Journal of Biological Engineering

Article Title: Rapid osteoinduction of human adipose-derived stem cells grown on bioactive surfaces and stimulated by chemically modified media flow

doi: 10.1186/s13036-025-00491-2

Figure Lengend Snippet: Fluid shear stress strengthens the osteogenic effects of rhBMP-2, PD98059 and Phenamil in ASCs cultured on SBG-PLGA composites. mRNA levels of osteoblastic markers after 7-day ASC culture on SBG-PLGA composites in ( A ) standard osteogenic medium under either static conditions or with fluid shear stress; and ( C ) osteogenic medium supplemented with 100 ng/ml rhBMP-2, 50 µM PD98059 and 20 µM Phenamil under either static conditions or with fluid shear stress. Results are presented as relative mRNA expression levels compared to mRNA levels in a control, static culture on PLGA (marked as a black line at 1). ( B ) The method of fluid shear stress application in ASC cultures using a standard laboratory see-saw rocker (7° tilt angle, 6 RPM frequency). ( D ) F-actin distribution in ASCs (Phalloidin-Atto488, magenta colored) at day 3 of culture in osteogenic medium supplemented with rhBMP-2, PD98059 and Phenamil after continuous static or dynamic culture conditions applied for 3 days. Scale bar represents 100 μm. ( E ) Western blot (WB) analysis of p-ERK1/2 and p-SMAD1/5/8 in ASCs after 1-h treatment with rhBMP-2 or rhBMP-2, PD98059 and Phenamil in static or dynamic conditions (upper panel) along with densitometric quantifications of WB results normalized to GAPDH levels. Averages ± SD are indicated. Two-way ANOVA test, * p < 0.05, ** p < 0.001, *** p < 0.0001 relative to the static PLGA control or between marked groups

Techniques: Shear, Cell Culture, Expressing, Control, Western Blot

Zinc (ZnO) or strontium (SrO) modified SBG-PLGA composites combined with fluid shear stress and BMP-based chemical stimulation, further increase osteogenic markers expression in early ASC cultures. mRNA levels of osteoblastic markers in ( A ) 3-day and ( B ) 6-day osteogenic ASC cultures on PLGA-based composites containing either unmodified or SrO- or ZnO-modified SBGs. Cells were treated with a combination of rhBMP-2, PD98059 and Phenamil at the indicated culture times in either static cultures or under fluid shear stress. Upper panels show the schemes of the ASC treatments. Results are presented as relative mRNA expression levels vs. mRNA levels in a control, static culture on PLGA (marked as a black line at 1). ( C ) Western blot (WB) analysis of phospho-β-catenin(Ser552), COX-2 and phospho-CREB(Ser133) levels in ASCs after 1-h treatment with rhBMP-2 or rhBMP-2, PD98059 and Phenamil in static or dynamic conditions (left panel) along with densitometric quantifications of WB results normalized to GAPDH levels (right panel). ( D ) Hypothesized signaling pathways involved in osteogenic response to treatment strategy. Averages ± SD are indicated. Two-way ANOVA test, * p < 0.05, ** p < 0.001, *** p < 0.0001 relative to the respective static PLGA control group or between marked groups

Journal: Journal of Biological Engineering

Article Title: Rapid osteoinduction of human adipose-derived stem cells grown on bioactive surfaces and stimulated by chemically modified media flow

doi: 10.1186/s13036-025-00491-2

Figure Lengend Snippet: Zinc (ZnO) or strontium (SrO) modified SBG-PLGA composites combined with fluid shear stress and BMP-based chemical stimulation, further increase osteogenic markers expression in early ASC cultures. mRNA levels of osteoblastic markers in ( A ) 3-day and ( B ) 6-day osteogenic ASC cultures on PLGA-based composites containing either unmodified or SrO- or ZnO-modified SBGs. Cells were treated with a combination of rhBMP-2, PD98059 and Phenamil at the indicated culture times in either static cultures or under fluid shear stress. Upper panels show the schemes of the ASC treatments. Results are presented as relative mRNA expression levels vs. mRNA levels in a control, static culture on PLGA (marked as a black line at 1). ( C ) Western blot (WB) analysis of phospho-β-catenin(Ser552), COX-2 and phospho-CREB(Ser133) levels in ASCs after 1-h treatment with rhBMP-2 or rhBMP-2, PD98059 and Phenamil in static or dynamic conditions (left panel) along with densitometric quantifications of WB results normalized to GAPDH levels (right panel). ( D ) Hypothesized signaling pathways involved in osteogenic response to treatment strategy. Averages ± SD are indicated. Two-way ANOVA test, * p < 0.05, ** p < 0.001, *** p < 0.0001 relative to the respective static PLGA control group or between marked groups

Techniques: Modification, Shear, Expressing, Control, Western Blot, Protein-Protein interactions

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